. Author manuscript; available in PMC: 2009 Jul 1.

Published in final edited form as: Blood Cells Mol Dis. 2008 Mar 10;41(1):22–34. doi: 10.1016/j.bcmd.2008.01.002

Table 1.

DIDS-sensitive and -insensitive whole-cell Cl^- currents recorded by nystatin-permeabilized patch clamp of red cells from Ae1^+/+ and Ae1^-/- mice.

Nystatin-permeabilized whole cell patch currents of red cells from ae1^+/+ and ae1^-/- mice.

Mouse Strain	Current (pA)		Normalized Current (pA/pF)
	@-100 mV	@+100 mV	@-100 mV	@+100 mV
ae1^+/+ (n=8)
- DIDS	-50±25	92±46	-20.5±6.9	38.3±13.4
+DIDS	-25±15 (p=0.03)	65±43 (p=0.01)	-11.5±4.5 (p<0.02)	22.0±7.2 (p=0.05)
% inhib	45±10%	42±11%	45±10%	40±9%
ae1^-/- (n=8)
-DIDS	-11.4±5.4	12.7±5.1	-6.7±2.9	12.0±6.6
+DIDS	-10.9±5.3 (n.s.)	12.9±4.5 (n.s.)	-10.2±4.8 (n.s.)	19.0±9.3 (p<0.05)
% inhib	4%	0%	0%	0 %

Values are presented as means + s.e.m.; p values are for two-tailed t tests assessing effects of DIDS.

Capacitance of AE1 +/+ cells (n=8) was 2.53±0.72 pF and 2.25±0.65 pF in absence and presence of DIDS, respectively.

Capacitance of AE1 -/- cells (n=8) was 1.73±0.47 pF and 1.06±0.36 pF in absence and presence of DIDS, respectively.