Figure 6.
MEHP (A) and 15d-PGJ2 (B) induce translocation of Bax to the mitochondria. Suspension cultures of BU-11 cells were treated with ethanol:DMSO (50:50, Vh, 0.1%), MEHP (150 μM) or 15d-PGJ2 (10 μM) for the times indicated. Cytoplasmic and mitochondrial fractions were prepared from digitonin-permeabilized cells and analyzed for Bax expression by immunoblotting. Representative data from at least 3 independent experiments are presented.