MEHP (A) and 15d-PGJ2 (B) induce Bid cleavage. Suspension cultures of BU-11 cells were treated with ethanol:DMSO (50:50, Vh, 0.1%), MEHP (150 μM) or 15d-PGJ2 (10 μM) for the times indicated. Cytoplasmic extracts were prepared and analyzed for full length (22 kDa) and cleaved Bid (15 kDa) by immunoblotting. Expression levels of full-length Bid were quantified as described in the Methods. Data are presented as means ± SE from at least 3 independent experiments. *Statistically less than Vh-treated (p<0.05, ANOVA, Dunnett’s).