Figure 3.

Transcription from the cad promoter is activated by the transactivation domain of Myc but not USF1. Asynchronously growing NIH 3T3 cells were transiently cotransfected with 0.5 μg of cadG4luc reporter plasmid and 2.5 μg of Gal4-Myc, Gal4-USF1, and Gal4-TFE3 expression plasmid. The cells were harvested for luciferase activity at 48 hr after transfection. For each experiment, activation of the cad reporter by the Gal4-fusion proteins was normalized to the activity of the Gal4 DNA-binding domain on the same reporter. Average activation and SEM was calculated from seven to 10 independent experiments by using multiple DNA preparations.