Figure 4.

Myc binds to the human cad and dhfr promoters in vivo. Crosslinking analysis of Myc, Max, and E2F4 binding at the human cad and dhfr promoters in log phase HeLa cells. Equivalent amounts of formaldehyde-crosslinked chromatin from asynchronously growing HeLa cells was incubated with antibodies to Myc, Max, E2F4, or in the absence of antibody (none). Immunoprecipitation of the human cad and dhfr promoters by each antibody was analyzed in parallel PCR reactions by using primers specific to the cad or dhfr promoters. The input sample (input) contains 0.02% of the total input chromatin as PCR template for each set of primers.