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. 1998 Nov 10;95(23):13887–13892. doi: 10.1073/pnas.95.23.13887

Figure 5.

Figure 5

Transcriptional activation by Myc is dependent on the context of Myc binding. (A) The transactivation domain of Myc cannot activate expression from the dhfr promoter from the distal upstream position. Asynchronous NIH 3T3 cells were transiently cotransfected with 2.5 μg of dhfrG4luc or −375G4dhfrluc reporter and 5.0 μg of Gal4-Myc expression plasmid. Cells were harvested for luciferase activity 48 hr after transfection. For each experiment, activation of the dhfr reporter by Gal4-Myc was normalized to the activity of the Gal4 DNA-binding domain on the same reporter. Data presented was obtained from five to seven independent experiments, using multiple DNA preparations. (B) Activation of cad expression is less sensitive to the position of Myc binding. Asynchronous NIH 3T3 cells were transiently cotransfected with 0.5 μg of cadG4luc or −335cadG4luc reporter and 2.5 μg of Gal4-Myc expression plasmid. Cells were harvested and analyzed as described above. Data presented was obtained from eight to nine independent experiments.