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Steady-state kinetic parameters for aminoacylation WT SepRS
| Phosphoserine | Phosphothreonine | Serine | Glutamate | |
|---|---|---|---|---|
| Plateau aminoacylation (%) | 72.7 ± 10 | 27.2 ± 5 | 8.0 ± 2 | 9.5 ± 3 |
| kcat (s-1) | 0.45 ± 0.10 | (5.4 ± 0.2) × 10-3 | NDa | ND |
| Km (amino acid) (mm) | 0.04 ± 0.01 | 8.4 ± 4.0 | ND | ND |
| kcat/Km (amino acid) (s-1m-1) | 1.13 × 104 | 0.64 | ND | ND |
Plateau aminoacylation levels are too low to permit accurate determination of kinetic parameters.
