TABLE 2.

RNase H-based K_d values

RT enzyme	16-bp hybrid duplex		22-bp hybrid duplex
	Kd(RNase H)	FCa	Kd(RNase H)	FC
	nm	-fold	nm	-fold
WT	104 ± 16.7		18.8 ± 7.30
TAMs	188 ± 84.4	1.81	43.9 ± 15.3	2.34
TAMs/A360V	369 ± 137	3.54	63.1 ± 49.9	3.36
TAMs/N348I	919 ± 13.0	8.84	83.6 ± 38.9	4.45
TAMs/A360V/N348I	>1000	>>>	128 ± 94.7	6.81

^aFC, -fold change in K_{d(RNase H)} value of each mutant RT was compared with that of WT RT.