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A 12% SDS–PAGE gel stained with Coomassie Brilliant Blue showing the apparent homogeneity of the purified EhMGL1. Lane 1, E. coli soluble extract before IPTG induction; lane 2, E. coli soluble extract after IPTG induction; lane 3, an eluate from a GSTrap HP column after digestion with PreScission Protease; lane 4, the purified EhMGL1 after size-exclusion chromatography using a HiPrep16/60 Sephacryl S-300 column; lane M, molecular-weight markers (kDa).
