Table 1.
PCR primers and DHPLC conditions
| Exon | Primers | Fragment Size | DHPLC Temperature |
|---|---|---|---|
|
CDKN2A
Exon 1α |
F-CAGCACCGGAGGAAGAAAG
R- GCGCTACCTGATTCCAATTC |
351 bp | 65, 68, (69) |
|
CDKN2A
Exon 2 |
F- GGAAATTGGAAACTGGAAGC
R- GGAAGCTCTCAGGGTACAAATTC |
499 bp | 60, 65, 70 |
|
CDKN2A
Exon 3 |
F- CCATTGCGAGAACTTTATCC
R- TGGACATTTACGGTAGTGGG |
329 bp | 56, 62 |
|
CDKN2A
Exon 1β |
F- CACCTCTGGTGCCAAAGGGC
R- CCTAGCCTGGGCTAGAGACG |
351 bp | 61, 65, 69 |
| CDK4
Exon 2 |
F- GCTGCAGGTCATACCATCCT
R- ATCATCACACCCCACCTATAGG |
371 bp | 62 |