Table 1. Electrophysiological properties of Cav3.1, Cav3.3, and their deletion mutants.
Current Density | Activation | Inactivation | Po estimate | ||||||||||||
Gmax | sem | V0.5 | sem | k | sem | n | V0.5 | sem | k | sem | n | G/Q | sem | n | |
(nS/pF) | (mV) | (mV) | (mV) | (mV) | |||||||||||
Cav3.1 WT | 2.9 | ±0.3 | −44.6 | ±0.7 | 5.8 | ±0.2 | 19 | −77.7 | ±0.7 | −5.0 | ±0.2 | 15 | 0.33 | ±0.05 | 9 |
GD1–2 | 4.5 | ±0.8* | −62.2 | ±5.8** | 4.6 | ±0.2*** | 9 | −91.0 | ±1.6*** | −4.0 | ±0.1*** | 9 | 0.94 | ±0.19** | 6 |
GD3–5 | 6.0 | ±1.2** | −56.0 | ±1.2*** | 4.0 | ±0.3*** | 8 | −81.2 | ±1.5* | −4.2 | ±0.2* | 8 | 0.31 | ±0.03 | 7 |
Cav3.3 WT | 2.3 | ±0.3 | −40.2 | ±0.8 | 5.8 | ±0.2 | 17 | −68.6 | ±0.7 | −5.5 | ±0.5 | 9 | 0.16 | ±0.02 | 9 |
ID1–2 | 2.4 | ±0.5 | −51.6 | ±1.4*** | 5.9 | ±0.2 | 9 | −83.0 | ±1.1*** | −4.3 | ±0.2 | 6 | 0.32 | ±0.04*** | 6 |
ID3–5 | 1.6 | ±0.3 | −43.2 | ±1.5 | 6.6 | ±0.2* | 8 | −76.1 | ±1.7*** | −5.1 | ±0.2 | 5 | 0.17 | ±0.01 | 6 |
The Gmax and V0.5 of activation were determined from the I-V protocol, and therefore have the same number of cells (n) in each measurement. The G/Q ratio was calculated for each individual cell, and then averaged. Statistical significance is denoted with asterisks, where three asterisks indicates P<0.001, two for P<0.01, and one for P<0.05.