Figure 2.

Three-dimensional fluorescence images. The cytoskeleton of the cells within the tissue is visualized by FITC staining and serial two-dimensional sections were obtained with a confocal laser scanning microscope. Three-dimensional pictures were then stacked from all single pictures. In (a–c) three examples of the serial pictures obtained from the tissue formed in a square channel are shown at depth (c) 0, (b) −135 μm and (a) −470 μm, as well as (d) the combination of all single pictures. In (e–g) three examples of the serial pictures obtained from the tissue formed in a hexagonal channel are shown at depth (e) −485 μm, (f) −260 μm and (g) 0, as well as (h) the combination of all single pictures.