Figure 2.
hRFPL1,2,3 Genes Are Expressed during Human Neurogenesis In Vitro and In Vivo
(A) hRFPL1 and hRFPL2,3 expressions were determined by real-time PCR during human embryonic-stem-cells-derived neurogenesis and normalized to the cDNA level in fetal brain. Results are displayed as mean ± standard error (SEM). ∗∗∗p < 0.001 versus day 0 with one-way ANOVA followed by post hoc Tukey's test. hRFPL1 and hRFPL2,3 expressions were correlated to those of the neuronal marker GAD67 (R2 = 0.99, p = 0.003 for hRFPL1 versus GAD67; R2 = 0.98, p = 0.012 for hRFPL2,3 versus GAD67 with Pearson product moment correlation).
(B) hRFPL1 and hRFPL2,3 expressions were assessed in different structures of the developing brain and in adult neocortex by real-time PCR normalized to the level of expression observed in adult brain. The gender of the embryo (M, male; F, female) and its age in weeks are indicated in the parentheses. Results are displayed as mean ± standard error (SEM).