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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: J Pediatr Surg. 2008 Jun;43(6):1182–1190. doi: 10.1016/j.jpedsurg.2008.02.053

Figure 2. Endogenous HB-EGF promotes intestinal healing by restitution after I/R in mice.

Figure 2

A) Quantification of restitution as determined by the number of structurally incompetent villi. HB-EGF (−/−) mice (■) had a higher number of incompetent villi compared to HB-EGF(+/+) mice (□) at all time points. Three hours after reperfusion, HB-EGF (−/−) mice continued to have a significantly higher number of incompetent villi compared to HB-EGF (+/+) mice. *P<0.05.

B) Quantification of the mucosa-to-serosa unidirectional clearance of FITC-dextran as an index of functional gut barrier recovery using the Ussing system. Under basal conditions, there was no significant difference in FITC-dextran clearance between HB-EGF (+/+) (□) and HB-EGF (−/−) (■) mice. Three hours after reperfusion, HB-EGF (+/+) mice had a significantly lower clearance compared to HB-EGF (−/−) mice, indicating a better functional recovery of the gut barrier in wild-type mice. *p<0.05.