Figure 4.

Binding between Fcp1 and RAP74. (A) Various ³⁵S-labeled portions of yeast Fcp1 and human FCP1a, as indicated, were synthesized in rabbit reticulocyte lysates (input), applied to GST and GST-yRAP74 (649–735) microaffinity columns, and processed as described in Materials and Methods. Volumes corresponding to 5% of the input and 25% of each eluate were loaded on the gel. Only the region of each autoradiogram containing full-length in vitro synthesis products is shown. (B) Various ³⁵S-labeled portions of yeast Fcp1 and human FCP1a, as indicated, were chromatographed on GST and GST-hRAP74 (436–517) microcolumns. The columns were run and the eluates analyzed as described for A.