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A eukaryotic factor stimulates rExoY adenylate cyclase activity. rExoY (1.0 μM) was assayed for adenylate cyclase activity in the presence or absence of PNE from CHO cells for 30 min under standard conditions. ∗ indicates that PNE was heated to 100°C for 5 min prior to addition to the assay mixture. Calmodulin (CaM, 10 μM) or 50 μg/ml BSA did not stimulate rExoY adenylate cyclase activity.
