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Kinetics of cAMP accumulation in infected CHO cells. CHO cells were infected with PA103ΔexoUexoT∷Tc expressing either native ExoY or catalytically inactive ExoYK81M. At the indicated times, lysate fractions were prepared and assayed for cAMP. Values are the average of three wells and reported as pmols of cAMP per 144 μg of cellular protein per well.
