Table 1.

Elevation of cAMP levels in P. aeruginosa-infected CHO cells

P. aeruginosa strain	Plasmid	cAMP, pmol*	Morphology change†
Uninfected	NA	<0.5	−
PA103ΔexoUexoT∷Tc	pUCP	<0.5	−
PA103ΔexoUexoT∷Tc	pUCPexoY	136.9  ±  4.5	+
PA103ΔexoUexoT∷Tc	pUCPexoY K81M‡	<0.5	−
PA103popD∷Ω	pUCPexoY	<0.5	−
PA103ΔexoUexoT∷Tc	pUCPexoY	95.8  ±  16.8§	NA
PA103ΔexoUexoT∷Tc	pUCPexoY	<0.5¶	+

NA, not applicable. 

^*Data are reported as pmol of cAMP normalized to 417 μg of cellular protein per well. 

^†CHO cell morphology change is defined as the change from an elongated triangular shape to a rounded morphology. 

^‡K81M is a catalytically inactive mutant of ExoY. 

^§Cells were pretreated with cytochalasin D for 30 min prior to infection. 

^¶CHO cell lysate was treated with 5′:3′-cAMP phosphodiesterase prior to assaying for cAMP.