Figure 3.

Disulfide exchange studies indicate that MinLeu has a strong preference for a native-like fold. Reverse-phase HPLC chromatograms of equilibrium disulfide populations under native and denaturing (6 M guanidinium isothiocyanate) conditions are shown, with each disulfide species indicated. The relative populations of disulfide species under native and denaturing conditions, as well as that calculated for an unfolded polypeptide chain by using a random walk model, are indicated. The same results (±4%) were obtained at 4°C as at room temperature.