Skip to main content
. 1998 Nov 10;95(23):13905–13910. doi: 10.1073/pnas.95.23.13905

Figure 2.

Figure 2

Scheme demonstrating that inhibition of HIV-1 RT can be monitored in yeast. To demonstrate that the HART assay is an appropriate means to screen for inhibitors of HIV-1 RT in yeast, the HART assay was carried out with a previously characterized NNRTI (8 Cl-TIBO) and controls for the specificity of inhibition. The rationale for that experiment is shown. Specific inhibitors of HIV-1 RT should not inhibit the activity of Ty elements (Ty-his3AI) because reverse transcription is carried out by Ty RT. HART elements should be inhibited by 8 Cl-TIBO. HIV-1 RT variants (L100I and Y181C) are resistant to 8 Cl-TIBO, and HART elements carrying them should be active.