Table 1.

HIV-1 RT activity and inhibition of HIV-1 RT can be quantitatively assayed in yeast

Element	Relative frequency of histidine prototrophy with indicated inhibitor
	No inhibitor	8 Cl-TIBO						TBZ
		0	5 μm	10 μm	20 μm	40 μm	80 μm	0	5 μm	10 μm	20 μm	40 μm	80 μm
Ty	2.9 (±1.9)  ×  10−2	1.0	1.06	0.85	1.24	0.92	1.28	1.0	0.88	1.01	0.90	0.98	0.97
HART	1.1 (±0.8)  ×  10−3	1.0	0.35	0.25	0.11	0.05	0.02	1.0	0.30	0.25	0.14	0.04	0.01
L100I	4.4 (±3.2)  ×  10−4	1.0	0.93	1.13	0.98	0.86	0.55	1.0	0.71	0.64	0.25	0.23	0.12
Y181C	1.4 (±0.8)  ×  10−4	1.0	0.88	1.0	1.03	0.90	0.58	1.0	0.88	1.0	1.03	0.90	0.58

The frequency of RT-mediated histidine prototrophy was determined for Ty and hybrid Ty/HIV elements after brief induction of expression. The frequency of RT-mediated events is shown in the “No inhibitor” column. The frequency of RT-mediated histidine prototrophy was determined for Ty and hybrid Ty/HIV elements in the presence and absence of 8 Cl-TIBO and TBZ. The frequencies for each element-inhibitor combination are expressed relative to the frequency of the element in the absence of inhibtor (no inhibtor = 1.0).