Figure 3.
ALP1 interacts with cABL. (Left) Full-length cABL protein was produced in Sf9 cells labeled with [35S]methionine, and cell lysates were incubated with immobilized GST alone (lane 1), GST-ALP1 (lane 2), and anti-ABL antibody bound to protein A-Sepharose (PAS) beads (lane 3). Bound proteins were separated by SDS/PAGE and visualized by fluorography. (B) COS cells were cotransfected with the pSRα-cABL and pCGN-ALP1 expression vectors. Lysates were immunoprecipitated with preimmune serum (lane 1), anti-ALP (lane 2), and anti-ABL (lane 3) antibodies. Immunoprecipitated proteins were analyzed by Western blotting with an anti-ABL monoclonal antibody.