Figure 3.

Immunofluorescence staining in HeLa cells transfected with pCS3HsCdc7 and pCS3 control vector plasmids using 9E10 mAb. HeLa cells were grown on glass coverslips and transiently transfected with the indicated plasmids. After fixation, cells were incubated with the 9E10 mAb. Immunofluorescence staining was performed, and nuclei were visualized by staining with Hoechst dye 33258 as described in Materials and Methods.