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. 1998 Nov 10;95(23):13911–13916. doi: 10.1073/pnas.95.23.13911

Figure 2.

Figure 2

(A) Southern Blot of DNA extracted from Rep-transformed cell lines and hybridized to radiolabeled U94 DNA probe. The numbers 10, 1, and 0.1 indicate lanes containing, respectively, 10, 1, and 0.1 copy equivalents of U94 DNA. Rep-expressing lines RepCl2, RepCl3, and RepCl4 are shown in lanes A, B, and C, while the control cell line, N1, and parental JJhan cells are shown in lanes D and E. DNA was digested with HindIII to excise U94 from the vector as a 1.5-kb band. (B) Presence of U94 transcripts in Rep-expressing cell lines (2, 3, 4). RNA (no initial reverse transcription step) and cDNA (+RT) were amplified by PCR and analyzed by agarose gel. Lane C represents a control amplification reaction, performed by using 10,000 DNA molecules of U94 template.