Table 1.

Primers and conditions for amplification of HHV-6 genes (nested reaction)

ORF	Primer sequence (3′ → 5′)	Amplimer DNA	Size cDNA, bp	PCR conditions
U16/17	AGAACTGCAAATCGTTCCG CGTAGAACAGAAGACCGGC	401	315	1 min at 94°C, 1 min at 55°C, 1 min + 3 sec/cycle at 72°C for 35 cycles; 10 min at 72°C
U39	GGCATAAGGAAACATCATGT GTAGAGCAAACGTTTTCGAT	353	—
U31	GGAGAATCTTGTAAGTATATGGTC CTCGGACTCATAGATCTCATACTG	659	—	1 min at 92°C, 1 min at 58°C, 1 min at 72°C for 10 cycles; 1 min at 92°C, 1 min at 58°C, 1 min + 3 sec/cycle at 72°C for 20 cycles; 10 min at 72°C
U42	AGGTTTACCGCAGAGTTGCC CAAAACAACGCATCCGAGAC	404	—	1 min at 94°C, 1 min at 61°C, 1 min +3 sec/cycle at 72°C for 35 cycles; 10 min at 72°C
U89/90	TCGCACCAATTAAAAAAATCC TCAAAGAAATATTGGAAGAGAGGTC	531	424
U91	CAGACATTACACATTCATTTCA(C/G) CAACTGCACTGAAACCACTAC	385	283
U94	CCCATTGGAACTGTGGTCT TTCAGGGCTATGCACTCACC	388	—
U81	AAATTGGTGGACAACGATAG ATTCTGGAATCGAGCACTCT	184	—	1 min at 94°C, 1 min at 57°C, 1 min + 3 sec/cycle at 72°C for 35 cycles; 10 min at 72°C