Figure 3.

Transactivation by v-Jun-ER chimeras. Cells were cotransfected with a luciferase reporter plasmid containing the HTLV promoter together with RCAS expression vectors as indicated. Cells were treated with 2 μM estrogen (gray bars), 200 nM tamoxifen (black bars), or ethanol as solvent control (white bars). Luciferase activity was normalized to the protein content of the samples. The result of a typical experiment done in triplicate for each inducer is shown. The transfection was repeated three times with consistent outcomes.