Table 1. 18 protein samples selected for crystallization condition screening.

	Locus tag†	Function	PDB code	MW (kDa)	pI	Concentration (mgml1)	Buffer (protein solution)‡
A	TTHA1797	Probable amidase	2dc0	46520	5.91	10.82	(i)
B	TTHA0859	Uridylate kinase		25274	7.30	13.10	(i)
C	TTHA1431	Conserved hypothetical protein	2cz8	7748	5.77	38.58	(i)
D	TTHA1437	Transcription regulator, Crp family		23818	5.69	10.32	(ii)
E	TTHA0789	Putative glutaryl-CoA dehydrogenase	2eba	42780	6.31	32.02	(i)
F	TTHA0122	Manganese-containing pseudocatalase	2cwl	33333	5.34	10.68	(i)
G	TTHA1671	Adenylate kinase		20754	5.05	10.24	(i)
H	TTHB192	Hypothetical protein	1wj9	23724	10.79	5.27	(i)
I	TTHB029	Conserved hypothetical protein	2e67	29598	5.30	7.42	(i)
J	TTHA0735	Cytidine deaminase		13280	7.16	11.11	(i)
K	TTHA1056	Putative phosphoglucosamine mutase		46968	6.04	19.25	(i)
L	TTHA0895	Universal stress-protein family	1wjg	14756	5.14	20.02	(i)
M	TTHA1699	Conserved hypothetical protein	2cx5	16555	9.51	10.99	(i)
N	TTHA1281	Conserved hypothetical protein	2e6x	8137	5.26	11.77	(i)
O	TTHA0338	Conserved hypothetical protein	2cw5	26917	5.97	7.04	(i)
P	TTHA0890	Putative 3-hydroxyacyl-CoA dehydrogenase		84291	5.56	37.26	(i)
Q	TTHA1969	Chromosome-partitioning protein, ParB family		29741	10.15	14.54	(iii)
R	TTHA1623	Metallo--lactamase superfamily protein	2z1n	22299	5.03	9.44	(i)

^†Protein samples are arranged in decreasing order of crystallization success rate with the HTS-80 and sitting-drop VD (see Fig. 1 ▶).

^‡(i) 20mM TrisHCl pH 8.0, 1mM dithiothreitol (DTT); (ii) 20mM TrisHCl pH 8.0, 1mM DTT, 150mM NaCl; (iii) 20mM TrisHCl pH 8.0, 1mM DTT, 500mM NaCl.