Table 2. Robots, methods and conditions for crystallization condition screening.
| HTS-80 sitting-drop VD | Crystal Finder hanging-drop VD | TERA modified MB | TOPAZ microfluidic FID | |
|---|---|---|---|---|
| Protein solution | 1.0l | 1.0l | 0.5l | 0.7nl |
| Crystallization solution | 1.0l | 1.0l | 0.5l | 2.0nl |
| Reservoir (l) | 100 | 200 | ||
| Oil (l) | 15† | |||
| Cell (m) | 700 100 10 | |||
| Temperature (K) | 293 | 293 | 291 | 293 |
| Humidity (%) | 60 | 90 | ||
| Crystallization plate | 96-well sitting-drop plate (Corning, 3773) | 50-well custom-made plate (Ishikawajima Inspection and Instrumentation) | Microwell minitray, 72 wells (Nunc, 438733) | Topaz 4.96 screening chip (Fluidigm, TPZ-M-4.96) |
†
7:3 paraffin oil:silicone oil.