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. 2007 Dec;77(2-3):157–164. doi: 10.1016/j.eplepsyres.2007.09.005

Figure 1.

Figure 1

Intracellular MK-801 blocks postsynaptic NMDAr. (A) The traces are voltage clamp recordings of eEPSCs in a layer V neurone (at least eight responses averaged) evoked by electrical stimulation of afferent pathways at 0.05 Hz. The large eEPSC seen at a holding potential of −60 mV, is replaced by a weak slow eEPSC during perfusion with NBQX (10 μM) and bicuculline (30 μM). (B) Shifting the holding potential to +40 mV revealed a large slow eEPSC that was subsequently abolished by a period of repetitive depolarizing steps from −60 to −10 mV over a period of 5 min. (C) Whole cell voltage clamp records of sEPSCs in a different layer V neurone. The three traces are continuous. The patch pipette contained MK-801, and prior to the recordings illustrated, the neurone had been subject to a series of depolarizing voltage steps (as described above and in the text) for a period of 10 min. Addition of NBQX to the perfusion medium (during red bar) rapidly abolished all sEPSCs.