Figure 2. Longitudinal immunoscope study of Vβ T lymphocytes.

(A) A loss of polyclonality was found in the Vβ6b family at M+68 in peripheral blood (PB) and BM of P7. After the induction course of chemotherapy, quantitative amplification revealed a disappearance of the pathological clone. (B) In P10 blast cells, 2 clones were detected, Vβ5 and Vβ2, which disappeared 1 month after chemotherapy. (C) Immunoscope analysis of P5 cells (Vβ1, Vβ2, and Vβ23) indicated that 4 years after chemotherapy, the 3 clones displayed a gaussian distribution. (D) Immunoscope analysis of P4 cells indicated that the Vδ1 clone, which had been detected retrospectively 17 months before leukemia diagnosis, remained dominant at M+36, 2 months after chemotherapy. Percentages within graphs denote percent of the Vβ rearrangements.