Fig. 4.
Altered nucleolin staining detected at 4 h post infection. Vero cells were synchronously mock- or HSV-1(F)-infected. At 2, 4, and 8 hpi, cells were fixed/permeablized and immunostained with antibodies specific for VP22 and nucleolin at 4 hpi. Nuclei were visualized following Hoechst staining. Overlay images of Hoechst and nucleolin at 8 hpi show colocalization of altered nucleolin with marginalized chromatin in infected cells. Arrows mark a portion of VP22 staining which is circular and surrounds altered nucleolin. Magnification, 100×.