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. Author manuscript; available in PMC: 2009 May 30.
Published in final edited form as: J Mol Biol. 2008 Apr 7;379(2):261–272. doi: 10.1016/j.jmb.2008.03.072

Figure 1.

Figure 1

Protein-based or cell-based assays to evaluate mouse hTLR2-specific IgG responses after immunization. Sera from 3 TLR2−/− BL6 mice and 4 WT BL6 mice were harvested 7 days after the 5th boost. Serial 2-fold dilutions were assayed for binding against recombinant hTLR2 protein by ELISA (top) or against hTLR2-HEK cells by flow-cytometry (bottom). Both assays utilized labeled anti-mouse IgG as a secondary Ab to detected hTLR2-specific IgG binding. In the cell-based assay, hTLR2-specific binding was measured as the difference in MFI between hTLR2-HEK and control WT-HEK staining. Serum from non-immunized mice was used as a negative control (naïve).