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. Author manuscript; available in PMC: 2009 May 30.
Published in final edited form as: J Mol Biol. 2008 Apr 7;379(2):261–272. doi: 10.1016/j.jmb.2008.03.072

Table 1.

Relative efficiencies of protein-based versus cell-based selection and screening methods.

Initial screening-Raw phage Fingerprint Purified phage binding to cells scFv binding to cells
Selected on Screened by Number of clones screened Number of Unique clones Number of clones positive Number of clones positive Number of clones positive
Protein ELISA 96 21 (21%) 12/21 (57%) 0/12 (0%) No data
cells ELISA 154 19 (12%) 8/19 (42%) 7/8 (88%) 5/5 (100%)
Flow cytometry 168 78 (46%) 9/23 (39%) 8/9 (89%) 5/5 (100%)