Table 4.
Primer name | Primer sequence (5′ to 3′)a |
---|---|
P1F | TAGGATCCTTCTTTTTTTTTAATTTTTGTGCTA |
P1R | TTCCTCTAGAGAAGAGCTTAAAGTTAA |
P2F | TAGTTGGCTATATTGGGATCCAA |
P2R | CAGAGAATTCTTTATTTGAAAAAATAATTTTTTC |
P3F | ACAGGAATTCTTTTTTTTTAATTTTTGTGCTATTCT |
P3R | TTGAATTCTTTTTCAAATTCTTCAATATCTTGA |
P4F | TTGGGATCCAAAATCTAATACAAGT |
P4R | GGAAATCTTCCTTGAAGCT |
P5F | GCAGCTAATGTTGCAAATCTTTTC |
P5R | GCAGGTGCTGGCTGTTGA |
P6F | ATGCCTCTTCCGACCATCAAGCA |
P6R | GATCGCAGTGGTGAGTAACCATGCA |
The underlined sequences are restriction enzyme sites: BamHI sites (P1F and P4F), a XbaI site (P1R), and EcoRI sites (P2R, P3F and P3R).