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. 1969 Feb;97(2):550–556. doi: 10.1128/jb.97.2.550-556.1969

Molecular Basis of Transient Repression of β-Galactosidase in Escherichia coli

Bonnie Tyler 1, Boris Magasanik 1
PMCID: PMC249726  PMID: 4886283

Abstract

The molecular basis of transient repression of β-galactosidase by glucose was examined. This repression acted only at the level of transcription. Apparently, it was not mediated by the I-gene product. Analysis of single cells in a culture subjected to transient repression showed that essentially all cells initially experienced repression and later became gradually resistant to repression.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOEZI J. A., COWIE D. B. Kinetic studies of beta-galactosidase induction. Biophys J. 1961 Nov;1:639–647. doi: 10.1016/s0006-3495(61)86913-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ganesan A. K., Rotman B. Transfer and incorporation of genes controlling beta-D-galactosidase synthesis from Hfr and F' donors of Escherichia coli. J Bacteriol. 1966 Nov;92(5):1378–1382. doi: 10.1128/jb.92.5.1378-1382.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gilbert W., Müller-Hill B. Isolation of the lac repressor. Proc Natl Acad Sci U S A. 1966 Dec;56(6):1891–1898. doi: 10.1073/pnas.56.6.1891. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. JACOB F., MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318–356. doi: 10.1016/s0022-2836(61)80072-7. [DOI] [PubMed] [Google Scholar]
  5. KEPES A. KINETICS OF INDUCED ENZYME SYNTHESIS. DETERMINATION OF THE MEAN LIFE OF GALACTOSIDASE-SPECIFIC MESSENGER RNA. Biochim Biophys Acta. 1963 Oct 15;76:293–309. [PubMed] [Google Scholar]
  6. Kaempfer R. O., Magasanik B. Mechanism of beta-galactosidase induction in Escherichia coli. J Mol Biol. 1967 Aug 14;27(3):475–494. doi: 10.1016/0022-2836(67)90053-8. [DOI] [PubMed] [Google Scholar]
  7. LARK K. G., LARK C. Changes during the division cycle in bacterial cell wall synthesis, volume, and ability to concentrate free amino acids. Biochim Biophys Acta. 1960 Oct 7;43:520–530. doi: 10.1016/0006-3002(60)90474-1. [DOI] [PubMed] [Google Scholar]
  8. Loomis W. F., Jr, Magasanik B. Genetic control of catabolite repression of the lac operon in Escherichia coli. Biochem Biophys Res Commun. 1965 Jul 12;20(2):230–234. doi: 10.1016/0006-291x(65)90351-7. [DOI] [PubMed] [Google Scholar]
  9. MCFALL E., MANDELSTAM J. SPECIFIC METABOLIC REPRESSION OF THREE INDUCED ENZYMES IN ESCHERICHIA COLI. Biochem J. 1963 Nov;89:391–398. doi: 10.1042/bj0890391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Moses V., Prevost C. Catabolite repression of beta-galactosidase synthesis in Escherichia coli. Biochem J. 1966 Aug;100(2):336–353. doi: 10.1042/bj1000336. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Müller-Hill B. Suppressible regulator constitutive mutants of the lactose system in Escherichia coli. J Mol Biol. 1966 Jan;15(1):374–376. doi: 10.1016/s0022-2836(66)80234-6. [DOI] [PubMed] [Google Scholar]
  12. NAKADA D., MAGASANIK B. THE ROLES OF INDUCER AND CATABOLITE REPRESSOR IN THE SYNTHESIS OF BETA-GALACTOSIDASE BY ESCHERICHIA COLI. J Mol Biol. 1964 Jan;8:105–127. doi: 10.1016/s0022-2836(64)80153-4. [DOI] [PubMed] [Google Scholar]
  13. Paigen K. Phenomenon of transient repression in Escherichia coli. J Bacteriol. 1966 Mar;91(3):1201–1209. doi: 10.1128/jb.91.3.1201-1209.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Palmer J., Moses V. Involvement of the lac regulatory genes in catabolite repression in Escherichia coli. Biochem J. 1967 May;103(2):358–366. doi: 10.1042/bj1030358. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Palmer J., Moses V. Role of the regulator-gene product (repressor) in catabolite repression of beta-galactosidase synthesis in Escherichia coli. Biochem J. 1968 Jan;106(2):339–343. doi: 10.1042/bj1060339. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Prevost C., Moses V. Pool sizes of metabolic intermediates and their relation to glucose repression of beta-galactosidase synthesis in Escherichia coli. Biochem J. 1967 May;103(2):349–357. doi: 10.1042/bj1030349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. ROTMAN B. Measurement of activity of single molecules of beta-D-galactosidase. Proc Natl Acad Sci U S A. 1961 Dec 15;47:1981–1991. doi: 10.1073/pnas.47.12.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. ROTMAN B., ZDERIC J. A., EDELSTEIN M. Fluorogenic substrates for beta-D-galactosidases and phosphatases derived from flurescein (3,6-dihydroxyfluoran) and its monomethylether. Proc Natl Acad Sci U S A. 1963 Jul;50:1–6. doi: 10.1073/pnas.50.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. SYPHERD P. S., STRAUSS N. THE ROLE OF RNA IN REPRESSION OF ENZYME SYNTHESIS. Proc Natl Acad Sci U S A. 1963 Dec;50:1059–1066. doi: 10.1073/pnas.50.6.1059. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Steers E., Jr, Craven G. R., Anfinsen C. B. Comparison of beta-galactosidases from normal (i-o+z+) and operator constitutive (i-ocz+) strains of E. coli. Proc Natl Acad Sci U S A. 1965 Oct;54(4):1174–1181. doi: 10.1073/pnas.54.4.1174. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tyler B., Loomis W. F., Jr, Magasanik B. Transient repression of the lac operon. J Bacteriol. 1967 Dec;94(6):2001–2011. doi: 10.1128/jb.94.6.2001-2011.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]

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