Figure 2.
Effect of RNAi mediated silencing of dopa decarboxylase on melanization of CM-Sephadex beads in Anopheles gambiae. A frequency distribution for 5 categories of increasing strength of melanization is presented. Control mosquitoes were not injected with double stranded RNA. GFP mosquitoes were injected with dsGFP. Inset: ethidium bromide stained gel demonstrating the degree of knockdown as measured by RT-PCR. RPS7 is used as a calibration control, resulting in approximately equal transcript levels of this nontarget gene in both dsDDC and dsGFP treatments.