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. Author manuscript; available in PMC: 2008 Aug 6.
Published in final edited form as: Am J Physiol Gastrointest Liver Physiol. 2007 Dec 20;294(3):G627–G634. doi: 10.1152/ajpgi.00519.2007

Figure 3. Identification of different nNOS forms in varicosity extracts.

Figure 3

Left Panel; Varicosity extracts under non-denaturing conditions (on ice) immunoblotted with anti-nNOS1422–1433 antibody showed dimers and monomer at 320, 250 and 155kD. Heat treated (37° C) extract probed with the same antibody detected monomers at 155 and 135kD. Right Panel; The nNOSα splice variant was identified in the varicosity extract by anti-nNOS1–20 antibody in the cold condition that detected a 320kD dimer and a 155kD monomer band. Heat treated extract probed with anti-nNOS1–20 antibody detected only the monomer at 155kD. Note that both splice variants of nNOS, nNOSα and nNOSβ, are present in the gut nerve terminals.