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. Author manuscript; available in PMC: 2008 Aug 6.
Published in final edited form as: Cell Stem Cell. 2008 Jul 3;3(1):55–68. doi: 10.1016/j.stem.2008.04.004

Figure 7. Mesp1 Rapidly Induces Expression of Many Genes, Including Myocardin, which Is Required for Differentiation of Cardiomyocyte and SMC Lineages.

Figure 7

(A) A2lox.Mesp1 ESCs were differentiated in the presence of DKK1 without dox (Control) or with addition of dox (Mesp1) at day 2. Gene expression was assessed by microarray analysis at the indicated times (hrs) after doxcycyline addition. Normalized expression values were compared for 3-fold changes between time-matched samples that were either untreated or treated with dox. 592 genes satisfying the comparison criteria are shown using hierarchical clustering analysis. (B and C) A2lox.Mesp1 ESCs containing inducible Mesp1 (Mesp1), A2lox.Myocd DN ESCs containing inducible DN myocardin (Δ585) lacking the transactivation domain (DN Myocardin) (Wang et al., 2001), and A2lox.Mesp1-Myocd DN ESCs containing simultaneously inducible Mesp1 and DN myocardin (Mesp1 + DN Myocardin) were differentiated as in Figure S1B, and cells were harvested on day 4 (B) and day 6 (C). (B) Expression of PDGFRα and Flk1 was assessed by flow cytometry as in Figure 1B. (C) Expression of αSMA and cTnT was assessed by flow cytometry as in Figure 4B. The numbers shown indicate the percent of live cells present within each quadrant.