Table 1.
c-fos immunoreactivity in sampled brain areas
| Brain region | FST/Ovx | FST/E2 | Us/Ovx | Us/E2 |
|---|---|---|---|---|
| Allocortex | ||||
| Anterior cingulate cortex area 3 | 164.2 ± 10.5 | 116.0 ± 5.2* | 25.1 ± 5.7 | 19.7 ± 4.9 |
| Piriform cortex | 137.1 ± 12.9 | 128.6 ± 6.1 | 34.9 ± 5.3 | 19.8 ± 6.4* |
| Dorsopeduncular cortex | 69.9 ± 6.3 | 58.8 ± 5.2 | 7.4 ± 1.4 | 8.9 ± 1.7 |
| Infralimbic cortex | 139.0 ± 7.7 | 116.4 ± 8.8 | 51.4 ± 7.2 | 23.4 ± 2.5* |
| Lateral orbital cortex | 129.9 ± 11.1 | 77.6 ± 3.8* | 19.1 ± 4.6 | 9.6 ± 2.6* |
| Perirhinal cortex | 101.2 ± 8.6 | 88.0 ± 5.3 | 15.9 ± 1.6 | 19.6 ± 4.9 |
| Retrosplenial cortex | 182.1 ± 17.6 | 52.9 ± 5.9* | 13.3 ± 5.5 | 12.2 ± 4.3 |
| Isocortex | ||||
| Frontal cortex area 2 | 77.2 ± 11.5 | 67.9 ± 5.8 | 2.7 ± 1.2 | 5.9 ± 2.6 |
| Forelimb cortex | 143.5 ± 6.8 | 81.2 ± 12.0* | 4.3 ± 1.1 | 6.1 ± 1.9 |
| Hindlimb cortex | 133.9 ± 22.9 | 93.8 ± 14.7 | 3.1 ± 0.5 | 4.6 ± 1.3 |
| Parietal cortex area 2 | 198.1 ± 25.2 | 100.8 ± 10.5* | 19.0 ± 3.8 | 9.8 ± 2.0 |
| Temporal cortex area 3 | 136.5 ± 12.2 | 56.6 ± 4.7* | 19.4 ± 2.4 | 5.3 ± 1.0* |
| Subcortical telencephalon | ||||
| Tenia tecta | 71.4 ± 5.37 | 51.0 ± 3.7* | 11.8 ± 2.8 | 6.8 ± 2.2 |
| Anterior claustrum | 176.3 ± 4.8 | 136.5 ± 8.5* | 18.7 ± 6.2 | 22.0 ± 6.7 |
| Endopiriform n. | 78.4 ± 6.6 | 55.7 ± 3.5* | 16.0 ± 2.6 | 16.9 ± 4.6 |
| Anterior dorsomedial striatum | 45.7 ± 4.5 | 31.8 ± 3.2* | 8.3 ± 1.5 | 8.2 ± 2.1 |
| Posterior dorsomedial striatum | 41.9 ± 2.3 | 24.0 ± 3.7* | 11.0 ± 2.4 | 15.4 ± 2.2 |
| N. accumbens core | 35.7 ± 2.9 | 32.4 ± 2.7 | 3.4 ± 1.2 | 6.3 ± 1.7 |
| N. accumbens shell | 101.9 ± 8.0 | 76.6 ± 6.1* | 29.0 ± 3.8 | 30.6 ± 3.9 |
| Lateral septal n. | 163.8 ± 10.1 | 162.8 ± 12.5 | 68.9 ± 8.3 | 37.3 ± 4.1* |
| Medial septal n. | 58.1 ± 5.4 | 32.8 ± 3.2* | 16.3 ± 2.7 | 10.6 ± 2.4 |
| Bed n. stria terminalis | 54.0 ± 3.7 | 32.4 ± 3.4* | 9.7 ± 1.3 | 9.0 ± 2.2 |
| Amygdala | ||||
| Basolateral | 35.7 ± 2.3 | 24.3 ± 1.9* | 10.2 ± 2.4 | 7.1 ± 1.7 |
| Basomedial | 40.5 ± 3.8 | 43.2 ± 1.6 | 4.8 ± 1.3 | 5.7 ± 1.4 |
| Central | 12.1 ± 2.1 | 12.8 ± 2.4 | 9.8 ± 0.9 | 3.9 ± 1.1* |
| Cortical | 82.0 ± 9.2 | 80.0 ± 7.7 | 12.9 ± 1.9 | 5.9 ± 1.1* |
| Medial anterodorsal | 75.4 ± 6.4 | 39.4 ± 4.4* | 14.9 ± 3.0 | 10.9 ± 3.0 |
| Anteroventral | 26.4 ± 2.0 | 14.8 ± 1.1* | 7.4 ± 1.4 | 3.8 ± 0.7 |
| Posterodorsal | 54.5 ± 3.5 | 36.0 ± 2.8* | 10.5 ± 1.9 | 6.6 ± 2.0 |
| Posteroventral | 60.0 ± 8.4 | 43.5 ± 5.8 | 8.8 ± 1.3 | 4.6 ± 1.1* |
| Amygdalohippocampal area | 24.1 ± 2.1 | 21.3 ± 1.4 | 3.8 ± 0.8 | 2.5 ± 0.6 |
| Hippocampus dorsal CA1 | 22.4 ± 1.9 | 8.3 ± 1.5* | 1.3 ± 0.4 | 0.78 ± 0.4 |
| Dorsal CA3 | 16.3 ± 2.0 | 7.3 ± 0.9* | 2.9 ± 0.8 | 3.1 ± 1.7 |
| Dorsal dentate gyrus | 17.6 ± 1.7 | 19.0 ± 2.9 | 5.9 ± 0.6 | 7.2 ± 0.9 |
| Thalamus | ||||
| Centromedian n. anterior | 76.2 ± 6.2 | 33.4 ± 2.9* | 19.8 ± 3.1 | 10.1 ± 2.5* |
| Posterior | 84.4 ± 11.7 | 16.5 ± 2.0* | 2.8 ± 0.8 | 5.8 ± 1.8 |
| Lateral habenula | 34.7 ± 3.7 | 35.8 ± 2.8 | 9.3 ± 1.5 | 3.1 ± 1.0* |
| Paraventricular n. anterior | 138.1 ± 8.7 | 85.4 ± 9.5* | 55.4 ± 13.4 | 43.5 ± 10.4 |
| Posterior | 125.3 ± 6.6 | 104.1 ± 4.3* | 17.8 ± 4.0 | 12.9 ± 1.6 |
| Rhomboid n. | 89.8 ± 8.6 | 32.6 ± 2.8* | 19.4 ± 4.3 | 11.8 ± 3.5 |
| Hypothalamus | ||||
| Paraventricular n. | 192.9 ± 11.7 | 85.4 ± 9.5* | 11.6 ± 3.1 | 7.4 ± 1.4 |
| Lateral n. | 36.7 ± 3.3 | 22.6 ± 3.0* | 7.1 ± 1.8 | 4.8 ± 0.6 |
| Ventromedial n. | 43.7 ± 7.8 | 10.1 ± 1.6* | 4.6 ± 1.8 | 5.2 ± 0.5 |
| Dorsomedial n. | 93.1 ± 5.9 | 47.4 ± 4.0* | 13.3 ± 2.9 | 11.1 ± 2.1 |
| Dorsal hypothalamic area | 74.1 ± 3.1 | 66.0 ± 4.3 | 13.6 ± 1.5 | 7.4 ± 1.7* |
Numbers of c-fos immunoreactive cell nuclei (mean ± SEM) in brain areas from Ovx control and Ovx E2 rats subjected to the FST (FST/Ovx and FST/E2, respectively) and in the same areas from the control and E2 animals not subjected to the FST (Us/Ovx and Us/E2, respectively). ∗ indicate significant differences (P < 0.05) between the two hormonal conditions in each case.