Figure 6.

Intracellular localization of the C-terminal domain of prominin. CHO cells were stably transfected with the expression vector containing the mouse prominin cDNA. Intact cells at 4°C (27) (A and B) or paraformaldehyde-fixed, Triton X-100-permeabilized cells (C and D) were incubated with the mAb 13A4 (10 μg/ml) and the antiserum against the C-terminal domain (1:3000), followed by appropriate fluorescein- and rhodamine-conjugated secondary antibodies and double immunofluorescence analysis using confocal microscopy. Single optical sections at the level of the coverslip are shown. (Bars = 10 μm.)