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. 1969 Mar;97(3):1009–1011. doi: 10.1128/jb.97.3.1009-1011.1969

Preparation of Cell Walls and Protoplasm of Neisseria with the Ribi Cell Fractionator

John E Martin Jr a, William L Peacock Jr a, Gilbert Reising a, Douglas S Kellogg Jr a, Edgar Ribi a,1, James D Thayer a,2
PMCID: PMC249806  PMID: 4975741

Abstract

The varied pressures required for disruption of Neisseria gonorrhoeae and other species of Neisseria when the Sorvall-Ribi refrigerated cell fractionator is used in the preparation of cell walls and cellular protoplasm are reported. Optimal disruption pressure for the gonococcus was considerably less than that required for other members of the genus Neisseria. Pressures varied from 8,000 psi for N. gonorrhoeae F62, colony type 4, to 22,000 psi for the nonpathogenic Neisseria—N. sicca, N. flava, and N. catarrhalis. Representative electron photomicrographs are shown.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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