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. 1998 Nov 10;95(23):13947–13952. doi: 10.1073/pnas.95.23.13947

Figure 2.

Figure 2

BTX and brevetoxin binding to sodium channels with point mutations in the local anesthetic receptor site. (A) Scatchard analysis of binding of [3H]BTX-B in the presence of 10 μM RU51049 and 100 nM PbTx-1 for WT (closed squares), I1760A (closed triangles), F1764A (open squares), and Y1771A (open circles). Nonspecific binding was determined by the addition of excess unlabeled veratridine. The data were normalized with respect to maximum binding. (B) Averages of Kd values for BTX binding determined from three experiments. (C) Scatchard analysis of binding of [3H]PbTx-3. Nonspecific binding was determined in the presence of excess unlabeled PbTx-3. (D) Averages of Kd values for PbTx binding determined from three experiments. %B/F, Percent maximum bound (3H]BTX-B)/(Free [3H]BTX-B).