Figure 5.

Northern blot analysis of PVY immune plants generated by crossing Pro[s]1 with Pro[a/s]4. Total RNA was extracted from nontransgenic tobacco plants (NT), immune progeny plants 2, 5, 8, 9, 12, 15, and 19, and susceptible parent plants Pro[s]1 (S) and Pro[a/s]1 (A/S) before PVY inoculation (right) and 14 days after inoculation (left). The RNAs were run on 0.9% agarose gels, transferred to membranes, and probed for Pro sense mRNA (A) and Pro antisense mRNA (B). Equal loading of samples is apparent by the similar levels of ribosomal trapping of the probe above the mRNA bands.