Figure 2.

Effect of expression of αTTP on the secretion of α-tocopherol from McARH7777 cells. (A) Expression of αTTP by transformed McARH7777 cells. McARH7777 cells were stably transfected with an αTTP expression plasmid. After selection in medium supplemented with G418, surviving colonies were subcultured and the level of αTTP was assessed by Western blotting using an anti-αTTP polyclonal antibody. The transfected cells were named McA-TTP, and one of these transformants, named McA-TTP21, was tested. Lane 1, McARH7777; lane 2, McA-TTP21. (B) McARH7777 and McA-TTP21 cell monolayers were incubated with α-[¹⁴C]tocopheryl acetate in liposomes. After incubation for the indicated times, the amount of α-[¹⁴C]tocopherol in the cell (black) and medium (shaded) fractions were measured. (C) The amounts of α-[¹⁴C]tocopheryl acetate in the cell fraction were also measured.