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Purification of B. burgdorferi His6-LysRS. Protein purification was monitored by SDS/PAGE followed by staining with silver nitrate. Lane 1, S100 total protein extract from E. coli DH5α/pET15b-BBlysS; lane 2, S100 total protein extract from E. coli BL21(DE3)/pET15b-BBlysS following induction with isopropyl thiogalactoside; lane 3, purified His6-LysRS; and lane M, molecular mass standards (sizes shown in kDa). The predicted molecular mass of His6-LysRS is 63.2 kDa.
