Figure 6.

Chromosomal location of the lysS gene in Borrelia. Total undigested DNA from B. hermsii strain HS1 (lane 1), B. burgdorferi strain B31 (lane 2), B. garinii strains G2 (lane 3) and IP2 (lane 4), and B. afzelii strain H2 (lane 5) were separated by field inversion electrophoresis on an agarose gel and stained with ethidium bromide (EtBr). Uncut and HindIII-digested λ DNA were included as size standards, as indicated to the left. The positions of the borrelial circular plasmids (cp), linear chromosome (lc), and linear plasmids (lp) are identified at the right. Duplicate Southern blots prepared from the same gel were hybridized at low stringency (2× SSC, 45°C) with probes specific for the lysS (KRS) and flagellin (FLA) genes. The relative positions of the λ size standards are indicated to the left of each blot.