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. 1998 Nov 10;95(23):13965–13970. doi: 10.1073/pnas.95.23.13965

Figure 5.

Figure 5

The expression of Arabidopsis GLB1 gene is regulated by metabolites. (A) Light and sucrose increase levels of GLB1 mRNA. Total RNA (10 μg) from 16-day-old Arabidopsis plants dark-adapted (lanes 1–3) or light-grown (lanes 4–6) for 2 days was used for Northern blot analysis. During the dark or light treatment, plants were grown in media containing 0% sucrose (lanes 1 and 4), 3% sucrose (lanes 2 and 5), or 3% manitol (lanes 3 and 6). As a control, 18 S rRNA was detected on a replicate blot. (B) Amino acids reduce levels of GLB1 mRNA. The 16-day-old Arabidopsis plants grown in a normal day/night cycle were light treated for 48 hr in MS media containing: no sucrose, no amino acid (lane 1); 3% sucrose, no amino acid (lane 2); or 3% sucrose plus 0.4 mM Asn (lane 3), 3.4 mM Gln (lane 4), 3 mM Glu (lane 5). The 18S rRNA was detected on a replicate blot as a loading control.