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. 1998 Nov 10;95(23):13965–13970. doi: 10.1073/pnas.95.23.13965

Figure 6.

Figure 6

Phenotypic analysis of Arabidopsis PII overexpressors. (A) Western blot analysis of 50 μg of total proteins extracted from wild-type (WT, lane 1) or PII overexpressors (T3 homozygous lines 1, 4, 24, 25, 26, 27, 43, and 44; lanes 2–9). (B) The 10-day-old Arabidopsis wild-type (WT) and the representative PII overexpressor (line 24) grown on MS basal medium containing 3% sucrose without a nitrogen source accumulate high levels of anthocyanin. (C) Difference in accumulation of anthocyanin between 10-day-old Arabidopsis WT and PII-24 overexpressor. Plants were grown on MS basal medium containing 3% sucrose and 1 mM Gln as the only nitrogen source. (D) Anthocyanin content of WT and PII overexpressors (lines 1, 4, 24, 25, 26, 27, 43, and 44) grown on MS basal medium containing 3% sucrose, no nitrogen (gray bars) or on 3% sucrose plus 1 mM glutamine as the only nitrogen source (blue bars). Results are shown as means ± SEM from anthocyanin determination of four independent pools of 30 seedlings/bar.