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. 1997 Nov 11;94(23):12446–12451. doi: 10.1073/pnas.94.23.12446

Figure 2.

Figure 2

RT-PCR amplification of IR superfamily genes in salmon gill cartilage. Degenerate oligonucleotide primers were synthesized, which encoded conserved amino acid sequences in the tyrosine kinase domains of human IR, IGF-R, IRR, and c-ros as shown. Primer IR-8(sense) = 5′-TTQGGNATGGTNTAQGAPGG; primer IR-3(antisense) = 5′-GTCATNCCPAAPTCNCCNATQTT with N = G+A+T+C, P = G+A, Q = T+C. These primers were used to amplify 100-ng coho cartilage RNA that had been reverse-transcribed into cDNA for 40 cycles PCR, and the RT-PCR product was analyzed on a 1.4% agarose gel (lane 2). Lane 1 = QX174/HaeIII markers.