Figure 4.

Expanded partial sequence of SIR-1 cDNA. The sequence was obtained by performing RT-PCR on salmon gill cartilage RNA with primers IR-10 (sense sequence = 5′-AAPCCNTGGACNCAPTAQGC) and IR-3; a 1800-bp DNA fragment was isolated by agarose gel electrophoresis, subcloned into pGEM4Z/SmaI, and sequenced. The 36-bp sequence homologous to exon 11 found in the hIR gene is boxed, and the tetrabasic cleavage site separating the a and B subunits are underlined.